Fluorescence lifetime estimation method for incomplete decay
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(2015) Fluorescence lifetime estimation method for incomplete decay. This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published version. Copyright and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable, the material made available in SRO has been checked for eligibility before being made available. Copies of full text items generally can be reproduced, displayed or performed and given to third parties in any format or medium for personal research or study, educational, or not-for-profit purposes without prior permission or charge, provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. A new incomplete decay signal model is proposed to describe the incomplete decay effects in a time-correlated single-photon counting (TCSPC) based fluorescence lifetime imaging (FLIM) system. Based on this model, we modified a MUltiple SIgnal Classification (MUSIC) algorithm to eliminate the incomplete decay effects. Monte Carlo simulations were carried out to demonstrate the performances of the proposed approach. Simulations show that the proposed method is insensitive to the laser pulse rate and has a larger lifetime dynamic range compared with previously reported approaches. As far as we know, this new method is the first non-fitting method that can resolve incomplete decay effects for multi-exponential decays. Introduction: Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool that has been widely used in material sciences, biology, chemical analysis, diagnosis, etc. The fluorescence lifetime is the average time that the excited molecule stays at the excited state before dropping back to the ground state. It is sensitive to the microenvironment, but independent of the illumination intensity and probe concentration. Therefore it can be a robust indicator to probe physiological parameters such as pH, O2, Ca 2+ , viscosity, refractive index, glucose, etc. [1, 2]. When the detector in a time-correlated single-photon counting (TCSPC) FLIM system captures a photon emitted from fluorophores, the TCSPC module measures the time delay between the excited laser pulse and the detected photon [3]. This procedure is repeated and a fluorescence …
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A fluorescence lifetime estimation method for incomplete decay
A new incomplete decay signal model is proposed to describe the incomplete decay effects in a time-correlated single-photon counting (TCSPC) based fluorescence lifetime imaging (FLIM) system. Based on this model, we modified a MUltiple SIgnal Classification (MUSIC) algorithm to eliminate the incomplete decay effects. Monte Carlo simulations were carried out to demonstrate the performances of th...
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تاریخ انتشار 2017